Osmotic fragility testSpherocytosis - osmotic fragility; Thalassemia - osmotic fragility
Osmotic fragility is a blood test to detect whether red blood cells are more likely to break down.
How the Test is Performed
A blood sample is needed.
Venipuncture is the collection of blood from a vein. It is most often done for laboratory testing.
In the laboratory, red blood cells are tested with a solution that makes them swell. This determines how fragile they are.
How to Prepare for the Test
No special preparation is necessary for this test.
How the Test will Feel
When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing or a slight bruise. This soon goes away.
Why the Test is Performed
This test is done to detect conditions called hereditary spherocytosis and thalassemia. Hereditary spherocytosis makes red blood cells more fragile than normal. With thalassemia, some red blood cells are more fragile than normal, but more of them are less fragile than normal.
Congenital spherocytic anemia is a disorder of the surface layer (membrane) of red blood cells. It leads to red blood cells that are shaped like sph...
Thalassemia is a blood disorder passed down through families (inherited) in which the body makes an abnormal form of hemoglobin. Hemoglobin is the p...
A normal test result is called a negative result.
Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your health care provider about the meaning of your specific test results.
What Abnormal Results Mean
An abnormal result indicates 1 of these conditions:
- Hereditary spherocytosis
Veins and arteries vary in size from one person to another, and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight, but may include:
- Excessive bleeding
- Fainting or feeling lightheaded
- Hematoma (blood buildup under the skin)
- Infection (a slight risk any time the skin is broken)
Gallagher PG. Red blood cell membrane disorders. In: Hoffman R, Benz EJ Jr, Silberstein LE, Heslop HE, Weitz JI, Anastasi J, eds. Hematology: Basic Principles and Practice. 6th ed. Philadelphia, PA: Elsevier Saunders; 2013:chap 43.
Gallagher PG. Hemolytic anemias. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine. 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 161.
Review Date: 2/11/2016
Reviewed By: Todd Gersten, MD, Hematology/Oncology, Florida Cancer Specialists & Research Institute, Wellington, FL. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.