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CSF smear

Spinal fluid smear; Cerebrospinal fluid smear

 

A cerebrospinal fluid smear is a laboratory test to look for bacteria, fungi, and viruses in the fluid that moves in the space around the spinal cord and brain. Cerebrospinal fluid (CSF) protects the brain and spinal cord from injury.

How the Test is Performed

 

A sample of CSF is needed. This is usually done with a lumbar puncture, or a spinal tap.

The sample is sent to a laboratory. There, a tiny amount is spread on a glass slide. Laboratory staff then view the sample under a microscope. The smear shows the color of the fluid and the number and shape of cells present in the fluid. Other tests may be done to check for bacteria or fungi in the sample.

 

How to Prepare for the Test

 

Follow instructions on how to prepare for a spinal tap.

 

Why the Test is Performed

 

Your health care provider may order this test if you have signs of an infection that affects the brain or nervous system. The test helps identify what is causing the infection. This will help your provider decide on the best treatment

 

Normal Results

 

A normal test result means there are no signs of an infection. This is also called a negative result. However, a normal result doesn't mean that there is no infection. The spinal tap and CSF smear may need to be done again.

 

What Abnormal Results Mean

 

Bacteria or other germs found in the sample may be a sign of meningitis. This is an infection of the membranes covering the brain and spinal cord. The infection can be caused by bacteria, fungi, or viruses.

 

Risks

 

A laboratory smear poses no risk. Your provider will tell you about the risks of a spinal tap.

 

 

References

Karcher DS, McPherson RA. Cerebrospinal, synovial, serous body fluids, and alternative specimens. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 23rd ed. Philadelphia, PA: Elsevier; 2017:chap 29.

O'Connell TX. Cerebrospinal fluid evaluation. In: O'Connell TX, ed. Instant Work-Ups: A Clinical Guide to Medicine. 2nd ed. Philadelphia, PA: Elsevier; 2017:chap 9.

 
  • CSF smear

    CSF smear - illustration

    Cerebral spinal fluid (CSF) is a clear fluid that circulates in the space surrounding the spinal cord and brain. CSF protects the brain and spinal cord from injury by acting like a liquid cushion. CSF is usually obtained through a lumbar puncture (spinal tap). During the procedure, a needle is inserted usually between the 3rd and 4th lumbar vertebrae and the CSF fluid is collected for testing.

    CSF smear

    illustration

    • CSF smear

      CSF smear - illustration

      Cerebral spinal fluid (CSF) is a clear fluid that circulates in the space surrounding the spinal cord and brain. CSF protects the brain and spinal cord from injury by acting like a liquid cushion. CSF is usually obtained through a lumbar puncture (spinal tap). During the procedure, a needle is inserted usually between the 3rd and 4th lumbar vertebrae and the CSF fluid is collected for testing.

      CSF smear

      illustration

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      Review Date: 7/31/2016

      Reviewed By: Jatin M. Vyas, MD, PhD, Assistant Professor in Medicine, Harvard Medical School; Assistant in Medicine, Division of Infectious Disease, Department of Medicine, Massachusetts General Hospital, Boston, MA. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.

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